Anti-Green Fluorescent Protein Antibody說明書

Green Fluorescent Protein (GFP) is a naturally fluorescent protein originally derived from jellyfish. GFP has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors which have become useful and ubiquitous tools in transgenic experiments. Fluorescent proteins enable a wide range of applications where they have functioned as cell lineage tracers, reporters of gene expression, or as a measure of protein-protein interactions

Chicken Polyclonal

Recombinant GFP expressed in Escherichia coli

Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%,w/v) as an anti-microbial agent.

Mixture of IgY fraction and affinity-purified antibodies

Chickens were immunized with recombinant green fluorescent protein (GFP) emulsified in Freund’s adjuvant. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using GFP conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (30 µg/mL) and purified IgY (10 mg/mL), mixed with glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with sodium azide and then filter-sterilized.